Investigatory Project No:-18- Detection of Hydrogen peroxide (Adultrant) in different samples of milkअन्वेषी / खोजी / जांच परियोजना:-18-दूध के विभिन्न नमूनों में हाइड्रोजन पेरोक्साइड (मिलावट) का पता लगाना

1.Aim / Objective:- Detection of Hydrogen Peroxide in different samples of Milk

2 Apparatus:- 

A.Test Tube, 

B. Dropper

3.Reagents:-

A.Potassium Iodide

B.Starch

A.Preparation of reagents:-

APreparation of Solution of KI :-

Weigh 20 g of potassium iodide and dissolve it in distilled water to obtain a 100 ml solution.

B.Preparation of solution of starch:-

Take 1 g starch powder and make its paste in distilled water and than add  it dropwise in boiling distilled water .

Boil it for 2-3minuts by heating and make up the volume to 100 ml.

C.Now mix equal volume of Potassium iodide solution and starch solution to make final  reagent.

4.Theory:-

Hydrogen Peroxide is a preservative, but according to PFA rule it is not permitted to be added in milk, Therefore, if it is found, then milk is said to be adulterated and can not be used for drinking and other purpose.

Appearance of blue color with reagent, indicates the presence of hydrogen peroxide in the milk sample whereas unadultrated milk sample remain colorless.

Limit of detection for  this method is 0.004% (v/v) because Appearance of blue color in the adulterated milk samples occurs if concentration of H2O2 is clearly 0.004% (v/ v) and above.

4.Procedure:-

Take 1 ml of milk sample in a test tube and Add 1 ml of the potassium iodide-starch reagent into it and mix well. Observe the color of the solution in the tube.

5.Observation Table:-

S.No.	Sample collected from	Colour of sample with reagent	H2O2 present or not
1	Saras Dairy	Colourless or No colour	No
2	Local Dairy	Colourless or No colour	No
3	Milk from Mr ….	Colourless or No colour	No
4	Milk from Mr……..	Blue colour appear	Yes
5	Milk From Mr ….	Colourless or No colour	No

6.Result:- The milk sample …4……are adulterated with H2O2 whereas milk sample 1,2,3 and 5..are not adulterated with H2O2.